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Next-generation sequencing analysis Recovered sequences were trimmed, merged, and de-multiplexed using CLC Genomics Workbench version 24 (Qiagen)

If the water freezes at this stage, the bottle can burst.Thanks Things You'll Need Bucket or cooler Ice Tap water 2 cups (600 g) of rock salt 2 16.9 fluid ounces (0.50 L) unopened plastic bottles of purified or distilled water Ladle or large spoon Thermometer References About This Article To freeze water instantly, first, create a salt and ice water mixture by packing a bucket or cooler half full of ice and adding just enough water to allow the ice cubes to move

Upregulation of CHAC1 has been consistently observed in models of retinal damage, kidney and liver injury and heat shockinduced oxidative stress, often in tandem with ER stress (Ju et al., 2025

10.1007/s10787-019-00596-3 66 SanaA.SaleemR.FaiziS